Table 1 Specific primers for amplification of target genes and β-actin gene
Name | Sequences | Primer Length | Tm (°C) | Size (bp) |
|---|---|---|---|---|
β-actin AY550069.1 | 5′-TGCGGGACATCAAGGAGA-3′ (F) | 18 | 55 | 175 |
5′-AGGAAGGAGGGCGGAAGAG-3′ (R) | 20 | |||
NLRP3 JQ219660.1 | 5′-TGGATAGCGGCAAGAGT-3′ (F) | 17 | 47 | 145 |
5′-GCAGCCAGTGAGCAGAG-3′ (R) | 17 | |||
ASC CV877895.1 | 5′-GCCATAAACCGGGTTCCTGA-3′ (F) | 20 | 60 | 148 |
5′-CCCATCCACTCTTGGCCATT-3′ (R) | 20 | |||
Caspase-1 NM_214162.1 | 5′-GCCTTGCCCTCATAATCT-3′ (F) | 18 | 60 | 282 |
5′-ACATCTGGGACTTCTTCG-3′ (R) | 18 | |||
IL-18 AY450287.1 | 5′-GGATATGCCTGATTCTGACTGTT-3′ (F) | 19 | 50 | 100 |
5′-GATGGTTACTGCCAGACCTCTA-3′ (R) | 19 | |||
IL-1β NM_214055.1 | 5′-GCAGTGGAGAAGCCGATGA-3′ (F) | 19 | 62 | 223 |
5′-GGTGGAGAGCCTTCAGCAT-3′ (R) | 19 | |||
GSDMD AK394823.1 | 5′-CCCCTTCTACTTCCATGACACT-3′ (F) | 22 | 54 | 256 |
5′-CCTCCGTCACCACGAACAC-3′ (R) | 19 |