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Table 1 Specific primers for amplification of target genes and β-actin gene

From: Induction of macrophage pyroptosis-related factors by pathogenic E. coli high pathogenicity island (HPI) in Yunnan Saba pigs

Name Sequences Primer Length Tm (°C) Size (bp)
β-actin AY550069.1 5′-TGCGGGACATCAAGGAGA-3′ (F) 18 55 175
5′-AGGAAGGAGGGCGGAAGAG-3′ (R) 20
NLRP3 JQ219660.1 5′-TGGATAGCGGCAAGAGT-3′ (F) 17 47 145
5′-GCAGCCAGTGAGCAGAG-3′ (R) 17
ASC CV877895.1 5′-GCCATAAACCGGGTTCCTGA-3′ (F) 20 60 148
5′-CCCATCCACTCTTGGCCATT-3′ (R) 20
Caspase-1 NM_214162.1 5′-GCCTTGCCCTCATAATCT-3′ (F) 18 60 282
5′-ACATCTGGGACTTCTTCG-3′ (R) 18
IL-18 AY450287.1 5′-GGATATGCCTGATTCTGACTGTT-3′ (F) 19 50 100
5′-GATGGTTACTGCCAGACCTCTA-3′ (R) 19
IL-1β NM_214055.1 5′-GCAGTGGAGAAGCCGATGA-3′ (F) 19 62 223
5′-GGTGGAGAGCCTTCAGCAT-3′ (R) 19
GSDMD AK394823.1 5′-CCCCTTCTACTTCCATGACACT-3′ (F) 22 54 256
5′-CCTCCGTCACCACGAACAC-3′ (R) 19