Fig. 3

Microscopic findings in an adult female bare-faced curassow (Crax fasciolata) with polyostotic osteosarcoma. a The neoplasm exhibited infiltrative growth with cells organized in bundles interspersed by marked production of osteoid matrix and areas of mineralization. HE, 200X. b Cytologic features of malignancy characterized by a high nucleus:cytoplasm ratio, coarse aspect chromatin with evidence of nucleoli, being possible to observe binucleated cells. HE, 400X. c Evidence of type I collagen deposition synthesized by neoplastic cells. Picrosirius red under polarized light, 200X; Inset; strong vimentin immunostaining in neoplastic cells. d Metastatic neoplastic cell proliferation in the right kidney by compressing the tubes, altering the tissue architecture (arrow). HE, 200X