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Table 4 In-process test and cryopreservation criteria

From: Manufacturing and banking canine adipose-derived mesenchymal stem cells for veterinary clinical application

Specification Expected Methods
Donor virology (RV, CDV, ICHV, CPV and CCV) Negative Elisa Kit
Viability ≥ 90 % Trypan blue staining
Sterility No growth Microbiology culture
Mycoplasma Negative Culture
Endotoxin > 0.5 EU/mL Limulus Amebocyte Lysate
Phenotype (CD29, CD34, CD44, CD45 and CD90) ≥ 95 % CD29, CD34, CD90
≤ 2 % CD34, CD45
Flow cytometry
Differentiation potential Osteogenesis, adipogenesis Induction culture