Fig. 4
From: Molecular phylogenetics of a recently isolated goat pox virus from Vietnam
Plasmid cloning and confirmation of the plasmid DNA. The PCR using specific primers EP32(F-R) for P32 gene. -ve is negative control of uninfected goat primary testicular culture; Positive band of 0.9 - 1 kb using viral infected goat primary testicular cells (A); Purification PCR product (SP-PCR) showed a clear 0.9 kb single band (B); The ligated PCR product in vector and transformed into competent E.coli (VK, 0.9 kb) (C); Representative of positive colony was screened for confirmation of plasmid DNA (3.9 kb - vector) and inserted band (0.9 kb - P32 gene) (D); M: 100 bp DNA ladder