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Table 2 Effect of sampling time, challenge and age on frequency of severe intestinal lesions

From: Developing an experimental necrotic enteritis model in turkeys - the impact of Clostridium perfringens, Eimeria meleagrimitis and host age on frequency of severe intestinal lesions

Study variableStudy variable levelCP1EM dose2Hours after EM3ExperimentsNo. of birds (gut contents)4% score3OPG5
Hrs post97–9911.6–153,434 (10)< 3.0a0a
EM6121–14911.6–153,491 (20)29.7b5.8b
(3-wk-old)168–17111.6–153,438 (9)18.4ab5.0b
 123–12915–201,4,953 (16)64.2c6.3e
 145–14715–201,4,9759 (10)1.7d5.4e
 125110–2098 (2)12.5g4.6e
 145110–20915 (2)6.7g6.2f
(5-wk-old)125110–2098 (2)75.0c4.6e
 145110–20917 (2)76.5c6.1f
ChallengeNone00119–1711,2,3,4,550 (12)< 2.1a0a
(3-wk-old)EM800.25–20119–1711,2,3,4,577 (16)6.5a5.5b
 EM + CP910.25–20119–1711,2,3,4,5278 (43)15.5b5.6b
Turkey age3-wk-old110–20125–145923 (8)8.7c5.8e
 5-wk-old110–20125–145925 (8)76.0d5.6e
  1. 1Clostridium perfringens inoculation (0 = no, 1 = yes) status of lesion scored birds, 2 Number (in thousands) of inoculated Eimeria meleagrimitis oocysts per bird, 3 number of hours elapsed between Eimeria meleagrimitis inoculation and examination of turkeys, 4 Number of bird scored for intestinal lesions (number of pooled samples of intestinal contents examined for OPG), 5 Median log10 of oocysts per gram intestinal contents, based on samples of contents from posterior small intestine (from Meckel’s diverticulum to caeco-intestinal junction), caeca and colorectum from Eimeria meleagrimitis-inoculated turkeys, 6 Hours elapses between Eimeria meleagrimitis inoculation and examination for intestinal lesions, 7 OPG data from experiments 4 and 9, 8 Inoculated with Eimeria meleagrimitis, 9 Inoculated with Eimeria meleagrimitis and Clostridium perfringens
  2. a-bDunn’s test with Bonferroni adjustment of p-values for multiple comparisons, c-dPearson chi-square test, e-fKruskal-Wallis rank test, gFisher’s exact test. Different letters indicate significant (p < 0.05) difference