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Table 1 In vitro metabolism of canine platelets stored for 5 days (n = 22)

From: Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days

ParametersDay 1Day 3Day 5
Platelet count (× 1010/unit)7.48 ± 2.397.69 ± 2.397.42 ± 2.79
MPV (fL)11.5 ± 1.212.5 ± 4.513.2 ± 6.6*
PDW (fL)13.0 ± 7.914.0 ± 5.515.1 ± 2.0*
pH (22 °C)7.0 ± 0.46.4 ± 0.5*5.9 ± 0.7**
pO2 (mmHg)107.6 ± 32.2115.9 ± 31.0140.1 ± 21.8*
pCO2 (mmHg)43.7 ± 3.125.5 ± 2.5**9.5 ± 2.5**
Glucose (mmol/L)25.3 ± 2.318.8 ± 4.4**13.7 ± 5.6**
HCO3 (mmol/L)11.9 ± 1.73.5 ± 2.8**0.6 ± 1.2**
Lactate (mmol/L)0.7 ± 0.31.8 ± 0.8*2.8 ± 1.2**
ATP (μmol/1011platelets)1.7 ± 0.61.2 ± 0.3*1.0 ± 0.3**
CD61 (%)91.5 ± 4.893.4 ± 4.391.8 ± 5.7
CD62P (%)2.7 ± 1.52.6 ± 3.62.6 ± 2.2
LDH (U/L)237.9 ± 159.1404.7 ± 98.5630.0 ± 162.1
Annexin V (%)2.4 ± 2.51.6 ± 1.13.5 ± 4.2
Caspase (%)10.2 ± 11.46.7 ± 5.912.0 ± 13.9
∆Ψm JC-1 (%)91.8 ± 3.984.2 ± 20.555.4 ± 37.3**
  1. Data are shown as mean ± SD.
  2. ΔΨm mitochondrial membrane potential, ATP adenosine triphosphate, HCO3 bicarbonate, LDH lactate dehydrogenase, MPV mean platelet volume, pCO2 partial carbon dioxidepressure, PDW platelet distribution width, pO2 partial oxygen pressure.
  3. ANOVA with Duncan’s test.
  4. * significantly different values between day 3 or 5 of storage compared to day 1 (p < 0.05). ** p < 0.001