Primers used for PCR-direct sequencing
|
Primers
|
sense
|
5′-
|
AGTCCCACCTTCAAAAATCC
|
−3’
|
385
|
antisense
|
5′-
|
AACTAAAAATGCAATTATCTTGAATG
|
−3’
|
Primers used for PCR-RFLP and PCR-directsequencing of FFPE samples
|
Primers
|
sense
|
5′-
|
TCTTTTGGCATTGTGTAAACTTG
|
−3’
|
156
|
antisense
|
5′-
|
CTTACATTTTCAGTTAAAGGGAGACT
|
−3’
|
Primers and probes used for Taq-Man duplex real-time PCR assay
|
Primers
|
sense
|
5′-
|
TTTAGTGAGATTCTGAAGTTGAGCATAATA
|
−3’
|
78
|
antisense
|
5′-
|
TCATTACTTCTTGCTGATCTTGACAA
|
−3’
|
Probe for Wild-type allele
|
5′-
|
(VIC)- CAATCTTTTTCCTTTTC-(MGB)
|
−3’
| |
Probe for Mutant allele
|
5′-
|
(FAM)-CAATCTTAATCCTTTTC-(MGB)
|
−3’
| |