Fig. 4

Neuropathology. a C-BSE. Florid plaques were visible at the level of the hippocampus. Mild spongiform change also was present in this region. Inset: magnified view of a florid plaque. Bar = 20 μm. b L-BSE. There was marked spongiform change present in the granule cell layer and at its interface with white matter of the cerebellum. Granule cell depletion was striking. c L-BSE. Magnified view of the location in image B demonstrating non-florid plaques in the white matter. d L-BSE. A non-florid plaque with hematoxylin and eosin staining characterized by amphophilic color and a fine, linear, crisscross pattern. Polarization of light through the non-florid plaque demonstrated birefringent dichroism