Table 3 Adenosine deaminase activity in animals with and without inflammatory conditions. Total adenosine deaminase (tADA), and isoenzymes ADA1 and ADA2, obtained in healthy animals and dogs with clinical leishmaniosis, horses with the acute abdominal disease, pigs with lameness and healthy cows before and after calving. Inflammation was assessed by the measurement of ferritin in dogs, serum amyloid A (SAA) in horses, C-reactive protein (CRP) in pigs, and haptoglobin (Hp) and white blood cell count (WBC) in cows. All results are expressed in the median (25th–75th percentiles). Units: IU/L (ADA), μg/L (ferritin), μg/mL (SAA and CRP), mg/L (Hp) and 10⁹/L (WBC). Unpaired Mann-Whitney test was used to compare tADA and isoenzymes results between healthy and diseased animals. The Wilcoxon signed-rank test was used to compare tADA and isoenzymes results between the two different measurements performed in cows

Dogs

Healthy

Canine leishmaniosis

Healthy

Canine leishmaniosis

N = 10

N = 10

N = 10

N = 10

 Ferritin

123.20 (106.60–145.00)

707.20*** (299.80–1483.00)

 tADA

12.90 (8.60–16.83)

9.05 (7.85–16.25)

4.25 (2.75–5.65)

10.60* (3.55–12.18)

 ADA1

12.12 (7.77–15.60)

8.78 (7.24–14.70)

2.81 (1.78–4.54)

7.49* (2.07–10.15)

 ADA2

1.00 (0.69–1.25)

0.61 (0.42–1.19)

1.16 (0.87–1.61)

2.11* (1.08–3.00)

Horses

Healthy

Acute abdominal disease

Healthy

Acute abdominal disease

N = 10

N = 10

N = 10

N = 10

 SAA

1.00 (1.00–1.00)

129.60*** (124.10–230.80)

 tADA

0.23 (0.16–0.35)

0.43 (0.01–0.90)

46.30 (18.74–61.48)

162.20* (47.00–379.30)

 ADA1

0.15 (0.05–0.20)

0.28 (0.01–0.52)

43.84 (18.26–60.80)

158.60* (42.36–368.80)

 ADA2

0.06^a (0.00–0.19)

0.01^a (0.00–0.51)

0.90 (0.26–2.50)

5.35** (2.02–10.53)

Pigs

Healthy

Lameness

Healthy

Lameness

N = 10

N = 10

N = 10

N = 10

 CRP

6.60 (3.73–13.78)

72.50*** (41.03–101.00)

 tADA

10.42 (8.34–13.70)

9.61 (6.86–10.47)

495.10 (347.00–823.40)

1829.00** (814.50–2683.00)

 ADA1

8.87 (7.11–12.53)

6.29* (4.07–7.91)

493.00 (346.40–822.40)

1823.00** (811.60–2677.00)

 ADA2

1.40 (0.79–1.91)

2.31* (1.45–4.36)

1.12 (0.55–2.06)

4.33*** (2.36–8.61)

Cows

Before calving

At calving

Before calving

At calving

N = 10

N = 10

N = 10

N = 10

 WBC

6.46 (5.76–7.55)

10.41** (9.03–13.58)

 Hp

0.00 (0.00–5.85)

4.82* (0.00–31.60)

 tADA

7.14 (6.01–8.21)

8.79* (6.93–13.47)

5.51 (2.93–6.96)

22.40** (13.19–28.61)

 ADA1

7.14 (6.01–8.21)

8.79* (6.93–13.47)

5.20 (2.55–6.57)

22.30** (12.63–28.40)

 ADA2

0.00^a (0.00–0.00)

0.00^a (0.00–0.00)

0.36 (0.13–0.46)

0.14 (0.02–0.29)