Fig. 5
From: Effects of physical and chemical factors on pseudorabies virus activity in vitro
Freeze-thaw cycle–titer curve of PRV. Note: 20℃ and 37℃ represent the thawing temperatures. The freezing temperature was − 80 °C, and two thawing temperatures were set: 20 °C in air and 37 °C in a water bath. The freeze-thaw cycles were repeated in this manner, and samples with a freeze-thaw number of 1, 5, 10, 15, 20, 25, 30, 35, and 40 were taken for titer measurement