Table 2 Treponema PCR results for swab cultures showing disinfectant efficacy where contact was made between hoof knives and BDD lesions. The effect of disinfectants on viable treponemes on hoof trimming knives (determined by PCR of cultures), swabbed before use, and again post-trimming and post-disinfection. In all samples knife-BDD lesion contact occurred during trimming
Disinfectant | PRE-TRIMMING | POST-TRIMMING | POST-DISINFECTION | |||
|---|---|---|---|---|---|---|
Treponema genus | BDD phylogroups | Treponema genus | BDD phylogroups | Treponema genus | BDD phylogroups | |
2% Virkon® n = 32 | 0/32 | 0/32 | 18/32 (56.3%) | 13/32 (40.6%) | 0/32 | 0/32 |
2% sodium hypochlorite n = 21 | 0/21 | 0/21 | 10/21 (47.6%) | 10/21 (47.6%) | 0/21 | 0/21 |
1:100 FAM30® n = 20 | 0/20 | 0/20 | 12/20 (60.0%) | 10/20 (50.0%) | 0/20 | 0/20 |
Water n = 38 | 0/38 | 0/38 | 24/38 (63.2%) | 14/38 (36.8%) | 0/38 | 0/38 |
Total (n = 111) | 0/111 | 0/111 | 64/111 (54.2%) | 47/111 (39.8%) | 0/111 | 0/111 |