Table 5 Primer sequences for selected genes used in RT-qPCR to validate the microarray data
Gene Symbol | Forward Primer Sequence | Reverse Primer Sequence |
|---|---|---|
ACTA2 | 5’CGGCTACTCCTTTGTGACG3’ | 5’CGTGGCCATCTCGTTCTC3’ |
HTR2B | 5’CCAATCCAGGCCAATCAAAG3’ | 5’CAGGTGATGTTGCTTGGGTT3’ |
TAGLN | 5’GACATGTTCCAGACCGTCGA3’ | 5’CAATGACGTGCTTTCCCTCC3’ |
ACTG2 | 5’TGCCAACAATGTCCTTTCCG3’ | 5’GCCTCCAATCCAGACTGAGT3’ |
SLIT3 | 5’CTGACAAGGACAACGGCATC3’ | 5’CCCATCATTCACCGTCTCCA3’ |
CDKN2A | 5’CATGTTGGCTCAGAATCGGG3’ | 5’CTCACGTCCAAGGCACAAAA3’ |
SLC10A6 | 5’GCTGTTGGATGGGTTTCTCA3’ | 5’TCCAAGAAAGCACCAGTCTCT3’ |
CILP | 5’TGCTCCAATTATACCGTGCG3’ | 5’CAGAACACTTGCTCCAGGGA3’ |
MMP12 | 5’GACACAATTCATGGACCCTGG3’ | 5’TCAAATACGTCAGGTCCTTGGA3’ |
ADAMTS5 | 5’GTTCCCAAATATGCAGGCGT3’ | 5’AGCTTCGAACCAATGATGCC3’ |
ADAMTS19 | 5′ GGACGGTGAGGTGTACTAAC 3’ | 5’ACTGCATTCCTTTACCACAGG 3’ |