Fig. 3

Volcano plots of differentially expressed genes comparing CKCS, non-CKCS, all diseased valves and normal dog valves. Red dots represent genes that show increased expression, green represent genes that show decreased expression and grey dots represent genes which did not pass the differential expression criteria. The X-axis shows fold change value and the Y-axis shows p-value. Central vertical line shows 0 fold change with negative fold changes on the left and positive fold changes on the right. Only fold changes of at least ±1.5 are shown. a CKCS vs Normal with FDR correction (q-value < 0.05). b non-CKCS vs CKCS with FDR correction (q-value < 0.05). c Non-CKCS vs Normal with no FDR correction (p-value < 0.05). d All diseased valves vs Normal with FDR correction (q-value < 0.05). The table shows numbers of DEG which met the stringent criteria (fold change ±1.5, FDR q-value < 0.05) are shown for each comparison