Table 3 PCR primers used to detect DNA extracted from the ticks taken from Xinjiang
From: Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang areas of China
Primers | Target gene | Primer sequence (5′ → 3′) | Annealing temp (°C) | Target fragment (bp) | Reference sequence |
|---|---|---|---|---|---|
F | IS1111 | GTGATCTACACGAGACGGGTT | 55 | 517 | M80806.1, KT391016.1, KT391020.1, KT391019.1, KT391018.1, KT391017.1, KT954146.1, KT391015.1, KT391014.1, KT391013.1, EU430257.1 |
R | CGTAATCACCAATCGCTTCGT | ||||
16S-Fw | 16S rRNA | TCGGTGGHGAAGAAATTCTC | 55 | 592 | KP994776.1, GU797243.1, KP994812.1, KP994826.1, KP994854.1, D89792.1, NR_104916.1, FJ787329.1, HM208383.1, AY342037.1, MH769217.1, MK182891.1 |
16S-Rv | AGGCACCAARTCATYTCTGACAAG |