Fig. 1

Flow cytometry and microscopy of NETs sourced from chronically S. aureus infected milk from 1428 and two Newbould infected cows (high and low somatic cell responders). Flow Cytometry of neutrophils isolated from infected milk was analyzed for surface expression of (a) myeloperoxidase and (b) CD62L (L-selectin). The red histogram depicts cell isolated from 1428 milk, dark gray is from a chronic Newbould challenged low SCC cow, and light gray represents a chronic Newbould challenged high SCC cow. Milk fat was additionally evaluated for the presence of Neutrophil Extracellular Traps (NETs). (c) 1428 S. aureus infected milk fat shows increased NET presence compared to both high (d) and low (e) SCC cows challenged with Newbould. Control NET staining shows DNAse treatment of 1428’s infected milk fat (f), and staining of clean healthy quarter milk fat from cow 1428