Skip to main content
Fig. 1 | BMC Veterinary Research

Fig. 1

From: PCV cap proteins fused with calreticulin expressed into polymers in Escherichia coli with high immunogenicity in mice

Fig. 1

The schematic structure (a) and SDS-PAGE (b) of four recombinant Cap-CRT fusion proteins. a Fragments were used for constructing the recombinant proteins. Blue squares represent completely cap of PCV2 and truncated calreticulin. GGGGS in grey are linkers between cap and calreticulin. Each fragment is encoded by BamHI and XhoI, respectively. b Solubility of rP4C, rC4P, rP5F and rF5P induced by IPTG at 37 °C. M: protein ladder; Lane 1,3,5,7: precipitate of pET-28a-rP4C/rC4P/rV5P/rF5P; Lane 2,4,6,8: supernatant of pET-28a-rP4C/rC4P/rV5P/rF5P

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us