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Table 5 The primers used in the detection of APR resistance genes and expected amplicon sizes

From: Wild-type cutoff for Apramycin against Escherichia coli

Gene DNA sequence (5′–3′) Product (bp) Reference
aac(3)-IV aac(3)-IV F
aac(3)-IV R 		TCGGTCAGCTTCTCAACCTT
GATGATCTGCTCTGCCTGTG 		314 [43]
npmA npmA F
npmA R 		CTCAAAGGAACAAAGACGG
GAAACATGGCCAGAAACTC 		641 [43]
apmA apmA F
apmA R 		CGTTTGCTTCGTGCATTAAA
TTGACACGAAGGAGGGTTTC 		656 [15]
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BMC Veterinary Research

ISSN: 1746-6148

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