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Table 5 Information relative to the inclusion and exclusion criteria of the variables considered in the dogs enrolled in the study

From: Profile of gamma-delta (γδ) T lymphocytes in the peripheral blood of crossbreed dogs during stages of life and implication in aging

  INCLUSION CRITERIA EXCLUSION CRITERIA
Informed consent from the owner Granted Not granted
Breed Mixed breed Pure breed
Health and Wellness Healthy life-style in close contact with the owners Less than 2 h of exercise each day
Not required for Puppy
Diet and Feeding Management Commercial standard pet food specific for each stage of life Regular meal time each day Immunonutrient supplementation (especially zinc ions)
Irregular feeding routine
Over- or under-feeding
Competitive eating
Nutritional Assessmenta Junior, Adult, Mature, Senior: BCS 4 or 5 out of 9 Puppy in poor body conditions
Vaccinations Regularly performed “vaccination plan” b (certified only by an electronic medical record) Any dogs with vaccination status not in line with inclusion criteria
Data not certified by an electronic medical record
Test performed after 3 weeks post-vaccination
Therapies, Trauma, Stressors or Imaging Diagnostic Techniques After 2 months from: Data not certified by an electronic medical record Immunomodulant or immunosuppressive therapies
Medications, surgeries, trauma and stressors
medications, surgeries, trauma and stressors
X-rays exposure or computed tomography (CT)
Sex Entire female or male Female: pregnant, in estrus, any pathological status of the reproductive tract
Male: cryptorchidism, any pathological status of the reproductive tract
Parasites Endo/ecto-parasites free Presence of endo/ecto-parasites or therapies in the last 3 weeks
Periodontal Diseasesc Stages 1, 2, 3 Stage 4 and/or other inflammatory/ulcerative diseases
Arthritis Joints had to be free from any pathological status Acute and chronic pathological conditions
  1. a A Body Condition Score (BCS) 9-point scale system according to the 2011 World Small Animal Veterinary Association (WSAVA) Nutritional Assessment Guidelines was adopted [37]: 1, 2, 3 = under ideal; 4, 5 = ideal; 6, 7, 8, 9 = over ideal
  2. b According to the 2011 American Animal Hospital Association (AAHA) Canine Vaccination Guidelines [38]
  3. c Periodontal disease was scored according to a periodontal 4-point scale system provided by the Merck Veterinary Manual [39]