Fig. 3
From: The first report of multidrug resistance in gastrointestinal nematodes in goat population in Poland
Polymorphism of the length of restriction fragments of variant-1 of the β-tubulin gene indicating the resistance of Trichostrongylus colubriformis to benzimidazoles (cropped gel). M – molecular-weight size marker pUC Mix (11–1444) (A&A Biotechnology, Gdańsk, Poland), L1-L5 – presence of gene fragment showing resistance for benzimidazoles (T. colubriformis), (1) – the first mixture with three single primers of a given species in ASA-PCR (Pc1 – forward primer, Pc4 – susceptible allele primer, Pc2 – reverse primer), (2) – the second mixture with three single primers of a given species in ASA-PCR (Pc1 – forward primer, Pc3 – resistant allele primer, Pc2 – reverse primer)