Fig. 4

Growth curves (a) and viability plots (b) for ASCs. a Growth curves for six canine ASCs before and after the induction of differentiation. At time point 1, cells were seeded and time point 2 represents the number of cells at the beginning of the treatment with pre-differentiation medium STIM1, which was applied for 24 h. Cell numbers increased after 3 days of differentiation (time point 3) but decreased again after 9 days of differentiation (time point 4), although differences in cell numbers were not statistically significant. b Average viability of male and female dogs derived ASCs before and after the induction of differentiation. The viability decreased in both male and female cells on day 9 of cell culture (^***P < 0.001), and there was statistically significant difference in viability between male and female cells (^##P < 0.01) at this time point