A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum

Plut, Jan; Jamnikar-Ciglenecki, Urska; Golinar-Oven, Irena; Knific, Tanja; Stukelj, Marina

doi:10.1186/s12917-020-02489-y

BMC Veterinary Research

Table 1 Real-time PCR and conventional PCR results in group samples on farms where PCV3 was detected

From: A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum

			*pig category*														*Total samples by farm (pos./tested)*^a
			5w		7w		9w		11w		13w		fatteners		b. sows		OF	sera (pos./all)
			5w		7w		9w		11w		13w		fatteners		b. sows		faeces	sera (pos./all)
*farm no.*	1		34.95 +	2/10	34.5 +	3/10	37.44 -	0/10	36.54 +	0/10	38.19 -	0/10	37.84 -	0/10	33.68 +	5/10	7/7	10/70
	1		neg.	2/10	neg.	3/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	5/10	0/7	10/70
	2		neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	39.31 -	0/10	neg.	0/10	1/7	0/70
	2		neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	0/7	0/70
	4		30.39 +	4/10	32.52 +	4/10	38.34 -	0/10	neg.	0/10	32.07 +	2/10	neg.	0/10	31.85 +	3/10	5/7	13/70
	4		37.00 -	4/10	36.1 +	4/10	neg.	0/10	neg.	0/10	36.38 -	2/10	neg.	0/10	37.04 -	3/10	4/7	13/70
	5		37.72 -	0/10	33.54 +	0/10	39.16 -	0/10	38.23 -	0/10	neg.	0/10	38.11 -	0/10	39.7 -	0/10	6/7	0/70
	5		neg.	0/10	38.37 -	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	neg.	0/10	1/7	0/70
	6		27.96 +	3/8	N/A	N/A	33.42 +	3/9	37.95 +	2/10	36.41 -	0/10	35.32 +	0/10	37.49 +	0/10	6/6	8/57
	6		32.9 +	3/8	N/A	N/A	36.45 -	3/9	neg.	2/10	neg.	0/10	neg.	0/10	38.2 -	0/10	3/6	8/57
*Total samples/category*	OF	Sera (pos./all)	4/5	9/48	3/4	7/40	4/5	3/49	3/5	2/50	3/5	2/50	4/5	0/50	4/5	8/50	*25/34 (73.5%)*	*31/237 (13.1%)*
*Total samples/category*	faeces	Sera (pos./all)	2/5	9/48	2/4	7/40	1/5	3/49	0/5	2/50	1/5	2/50	0/5	0/50	2/5	8/50	*8/34 (23.5%)*	*31/237 (13.1%)*

The numerical value in OF/faeces cell represents a Ct value obtained with real-time PCR.
The symbol in the OF/faeces cell represents the result of conventional PCR testing (+ = positive, − = negative, [no symbol] = not tested),
The right part of the cell represents a number of positive individual sera compared to all tested sera with conventional PCR testing.
N/A = category not available for testing at the farm.
neg negative.
pos positive.
^aall the samples from Farm 3 are PCV3 negative farm are excluded from the table

Back to article page

ISSN: 1746-6148

Contact us

General enquiries: journalsubmissions@springernature.com