Impact of dietary zinc oxide nanoparticles on selected serum biomarkers, lipid peroxidation and tissue gene expression of antioxidant enzymes and cytokines in Japanese quail

Table 5 Oligonucleotide name, sequence and accession numbers of qRT-PCR primers

Gene	Sequence	Accession #	References
SOD1	F: TGGACCTCGTTTAGCTTGTG	XM_015881247.1	[50]
SOD1	R: ACACGGAAGAGCAAGTACAG	XM_015881247.1	[50]
CAT	F: CCTGACTATGGTGCGCGTAT	NC_029520.1	Designed
CAT	R: CAGACACACGAGAAGTGGCT	NC_029520.1	Designed
GPX1	F: CAG TTC GGG CAT CAG GAGAA	AB371709.1	[51]
GPX1	R: CGA GGA ACT TGC TCGAAA GTT ACC AGG	AB371709.1	[51]
GPX7	F: TTGTAAACATCAGGGGCAAA	XM_015870585.1	[50]
GPX7	R: TGGGCCAAGATCTTTCTGTAA	XM_015870585.1	[50]
IL-6	F: CAACCTCAACCTGCCCAA	XM_015853679.1	[52]
IL-6	R: GGAGAGCTTCCTCAGGCATT	XM_015853679.1	[52]
IFN-α	F: CCTTGCTCCTTCAACGACA	AB154298.1	[53]
IFN-α	R: CGCTGAGGATACTGAAGAGGT	AB154298.1	[53]
β-actin	F: CTGGCACCTAGCACAATGAA	XM_015876619.1	[54]
β-actin	R: CTGCTTGCTGATCCACATCT	XM_015876619.1	[54]

Abbreviations: qRT-PCR quantitative real time polymerase chain reaction, SOD1 super oxide dismutase-1, CAT catalase, GPX1 glutathione peroxidase-1, GPX7 glutathione peroxidase-7, IL-6 interleukin 6, IFN-α interfron α, β-actin beta actin

ISSN: 1746-6148