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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Bacillus subtilis spore vaccines displaying protective antigen induce functional antibodies and protective potency

Fig. 1

Expression and display of PAs. a SDS-PAGE and b Western blot images of PAs detached and decoated from spore surface. c Flow cytometry histogram for N spore (control spore), PA spore (PA-displaying spore), and PA-A spore (PA naturally displayed and adsorbed again on the spore). Abbreviation: M, Marker; N, N spore, B. subtilis spore; PA, Protective antigen displaying B. subtilis spore; Dt, NaCl treated detached protein; Dc, NaCl treated coat protein; Dtx, Triton X-100 treated detached protein; Dcx, Triton X-100 treated coat protein; Af, after adsorption; Ct, coat protein. Arrow, size of the PA protein

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