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Table 2 Positive samples in PEDV real-time RT-RPA method, real-time RT-PCR assay, and RT-PCR assay

From: Rapid differentiation of PEDV wild-type strains and classical attenuated vaccine strains by fluorescent probe-based reverse transcription recombinase polymerase amplification assay

Location in Gansu Province

Samples

qRT-PRA(a)

qRT-PCR(b)

RT-PCR(c)

Wild

PEDV

Vaccine

PEDV

Wild

PEDV

Vaccine

PEDV

wild PEDV

vaccine PEDV

DingXI

25

23

0

24

0

23

0

JiayuGuan

19

15

2

15

2

14

2

LinXia

23

17

3

17

3

16

2

TianShui

13

9

2

9

2

9

2

total

80

64

7

65

7

62

6

positive rate

80.00%%

8.75%

81.25%

8.75%

77.50%

7.50%

  1. a. Method established in this study
  2. b. Method from Su, et al. 2018 [31]
  3. c. Method from Zhao, et al. 2014 [32]