Rapid differentiation of PEDV wild-type strains and classical attenuated vaccine strains by fluorescent probe-based reverse transcription recombinase polymerase amplification assay

Table 1 The sensitivity and repeatability of established PEDV real-time RT-RPA method

Standard RNA	Copy number	Time (mean ± S.D.)^c	CV%	Standard RNA	Copy number	Time (mean ± S.D.)^c	CV%
N gene of PEDV CV777^a	3.0 × 10⁸	4.04 ± 0.041	1.01	ORF1 of PEDV CV777^b	3.0 × 10⁸	4.56 ± 0.067	1.47
	3.0 × 10⁷	5.31 ± 0.061	1.15		3.0 × 10⁷	5.87 ± 0.071	1.20
	3.0 × 10⁶	6.58 ± 0.075	1.14		3.0 × 10⁶	7.18 ± 0.087	1.21
	3.0 × 10⁵	7.85 ± 0.081	1.03		3.0 × 10⁵	8.49 ± 0.802	0.94
	3.0 × 10⁴	9.12 ± 0.076	0.83		3.0 × 10⁴	9.80 ± 0.068	0.69
	3.0 × 10³	10.39 ± 0.108	1.04		3.0 × 10³	11.11 ± 0.075	0.68
	3.0 × 10²	11.66 ± 0.096	0.82		3.0 × 10²	12.42 ± 0.093	0.75
	3.0 × 10¹	None	None		3.0 × 10¹	None	None
	3.0 × 10⁰	None	None		3.0 × 10⁰	None	None
	NCT	None	None		NCT	None	None

a. Related to the N gene of PEDV classical attenuated vaccine strain CV777, the Standard RNA as template was used in the PEDV universal real-time RT-RPA assay
b. Related to the ORF1 region of PEDV classical attenuated vaccine strain CV777, the Standard RNA as template was used in the PEDV vaccine real-time RT-RPA assay
c. The reaction procedure was performed by a total of 60 cycles in the real time RT-RPA method (20 s per cycle)

ISSN: 1746-6148