Fig. 1

ITS sequence-based phylogenetic tree. Phylogenetic relationship between the known (GenBank) and identified (this study) E. bieneusi genotypes were identified through an NJ analysis based on the Kimura two-parameter model. Sequence detection was performed based on its host origin, accession number, and the designated genotype. The branches show the percent bootstrapping values from 1000 replicates. Outgroup classification comprised of the E. bieneusi genotype CSK2 (KY706128) from white kangaroo. The filled triangles and squares indicate known and novel genotypes, respectively