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Table 5 Clinical performance of triplex real-time PCR and conventianal PCR

From: Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus

Virus

Triplex real-time PCR asssay

TP a

FN

TN

FP

Tatal

Sensitivity

(%)

Specificity(%)

Positive predictive value(%)

Negative predictive value(%)

AIV

16

0

103

1

120

100.00

99.04

94.12

100.00

NDV

6

0

113

1

120

100.00

99.13

85.71

100.00

DTMUV

3

0

37

0

40

100.00

100.00

100.00

100.00

Virus

Conventional PCR methods

TPa

FN

TN

FP

Tatal

Sensitivity(%)

Specificity(%)

Positive predictive value(%)

Negative predictive value(%)

AIV

13

3

99

5

120

81.25

95.19

72.22

97.06

NDV

5

1

112

2

120

83.33

98.25

71.43

99.12

DTMUV

3

0

37

0

40

100.00

100.00

100.00

100.00

  1. a TP: true positive, FN: false negative, TN: true negative, FP: false positive