Table 4 Results of the clinical positive samples detected by triplex real-time PCR, conventional PCR and virus isolation
Positive samples | AIV | NDV | DTMUV | ||||||
|---|---|---|---|---|---|---|---|---|---|
triplex real-time PCR | Conventional PCR | Virus isolation | triplex real-time PCR | Conventional PCR | Virus isolation | triplex real-time PCR | Conventional PCR | Virus isolation | |
1 | + | + | + | – | – | – | – | – | – |
2 | + | + | + | – | – | – | – | – | – |
3 | + | + | + | – | – | – | – | – | – |
4 | + | + | + | – | – | – | – | – | – |
5 | + | + | + | – | – | – | – | – | – |
6 | + | + | + | – | – | – | – | – | – |
7 | + | + | + | – | – | – | – | – | – |
8 | + | + | + | – | – | – | – | – | – |
9 | + | + | + | – | – | – | – | – | – |
10 | + | + | + | – | – | – | – | – | – |
11 | + | + | + | – | – | – | – | – | – |
12 | + | + | + | + | + | + | – | – | – |
13 | + | + | + | + | + | + | – | – | – |
14 | + | – | + | – | – | – | – | – | – |
15 | + | – | + | – | – | – | – | – | – |
16 | + | – | + | – | – | – | – | – | – |
17 | + | – | – | – | – | – | – | – | – |
18 | – | + | – | – | – | – | – | – | – |
19 | – | + | – | – | – | – | – | – | – |
20 | – | + | – | – | – | – | – | – | – |
21 | – | + | – | – | – | – | – | – | – |
22 | – | + | – | – | – | – | – | – | – |
23 | – | – | – | + | + | + | – | – | – |
24 | – | – | – | + | + | + | – | – | – |
25 | – | – | – | + | + | + | – | – | – |
26 | – | – | – | + | – | + | – | – | – |
27 | – | – | – | + | + | – | – | – | – |
28 | – | – | – | – | + | – | – | – | – |
29 | – | – | – | – | – | – | + | + | + |
30 | – | – | – | – | – | – | + | + | + |
31 | – | – | – | – | – | – | + | + | + |
Positive rates (%) | 14.17 (17/120) | 15.00 (18/120) | 13.33 (16/120) | 5.83 (7/120) | 5.83 (7/120) | 5.00 (6/120) | 7.50 (3/40) | 7.50 (3/40) | 7.50 (3/40) |