Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus

Table 3 The detection of the co-infection models by triplex real-time PCR

Co-infection proportion ^a	Number of DNA copies (copies/μL)			Co-infection real-time PCR Ct Value (mean ± SD) ^b
Co-infection proportion ^a	AIV	NDV	DTMUV	AIV	NDV	DTMUV
AIV:NDV:DTMUV = 10:1:1	1 × 10⁷	1 × 10⁶	1 × 10⁶	13.66 ± 0.13	18.15 ± 0.27	18.15 ± 0.27
AIV:NDV:DTMUV = 10:5:1	1 × 10⁷	5 × 10⁶	1 × 10⁶	13.50 ± 0.28	17.25 ± 0.27	18.03 ± 0.16
AIV:NDV:DTMUV = 1:5:10	1 × 10⁶	5 × 10⁶	1 × 10⁷	16.58 ± 0.22	17.13 ± 0.15	14.54 ± 0.28
AIV:NDV = 1:1	1 × 10⁷	1 × 10⁷	–	13.28 ± 0.48	14.43 ± 0.33	–
AIV:NDV = 10:1	1 × 10⁷	1 × 10⁶	–	13.57 ± 0.22	18.25 ± 0.23.	–
AIV:NDV = 100:1	1 × 10⁷	1 × 10⁵	–	13.93 ± 0.10	21.73 ± 0.38	–
AIV:DTMUV =1:1	1 × 10⁷	–	1 × 10⁷	13.43 ± 0.32	–	14.38 ± 0.34
AIV:DTMUV =10:1	1 × 10⁷	–	1 × 10⁶	13.57 ± 0.29	–	17.92 ± 0.32
AIV:DTMUV =100:1	1 × 10⁷	–	1 × 10⁵	13.24 ± 0.34	–	21.33 ± 0.18
NDV:DTMUV =1:1	–	1 × 10⁷	1 × 10⁷	–	14.32 ± 0.32	14.53 ± 0.22
NDV:DTMUV =10:1	–	1 × 10⁷	1 × 10⁶	–	14.49 ± 0.19	18.02 ± 0.14
NDV:DTMUV =100:1	–	1 × 10⁷	1 × 10⁵	–	14.54 ± 0.15	21.68 ± 0.23

^a There were two kinds of co-infection models included:the triplex co-infection and duplex co-infection
^b The data was shown as the mean ± SD (n = 3)
-: represents no design in the corresponding system

ISSN: 1746-6148