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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus

Fig. 4

Sensitivity of the conventional PCR method. Templates of pMD-AIV (A), pMD-NDV (B) and pMD-DTMUV (C) were diluted from 1 × 107 copies/μL to 1 × 101 copies/μL. The detection limit of conventional PCR is 1 × 102 copies/μL for AIV, NDV and DTMUV. The gels were cropped. M, DL2000 marker; NC, negative control.

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