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Fig. 6 | BMC Veterinary Research

Fig. 6

From: The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

Fig. 6

a. Gating strategy for flow cytometry data analysis. Granulocytes and monocytes were gated based on forward and side scatter (FSC/SSC) parameters. Each cell subset was analysed for the relative number of phagocytising cells and cells stimulated for respiratory burst (fMLP, PMA or E. coli bacteria). b. Gating strategy for analysing flow cytometry data based on the neutrophil migration assay. Granulocytes and counting beads were identified in PerCP to SSC scatter and depicted in FSC to SSC scatter. (a) – control without stimulation; (b) – control stimulated with fMLP; (c) – HMB without stimulation; (d) – HMB stimulated with fMLP

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BMC Veterinary Research

ISSN: 1746-6148

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