Fig. 5

a The percentage of monocytes stimulated to undergo respiratory burst in calf groups after stimulation with fMLP, PMA and E. coli, as determined in the Bursttest® kit. Key: I – control group; II – experimental group; SD - standard deviation. Numerical results were presented as the arithmetic mean ± SD. The significance level was set at 0.05. Asterisks refer to statistically significant differences between the control group and the experimental group on the same sampling day at * p < 0.05; ** p < 0.01; *** p < 0.001; B, C refer to statistically significant differences between day “0” and the consecutive sampling days in the experimental group at B – p < 0.01; C – p < 0.001. b. Dot plot cytogram showing the percentage of monocytes stimulated to undergo respiratory burst in control and experimental animals on experimental day 30. The percentages of monocytes stimulated to undergo respiratory burst were gated. c. Mean fluorescence intensity (MFI) of monocytes in calf groups after stimulation with fMLP, PMA and E. coli, as determined in the Bursttest® kit. Key: I – control group; II – experimental group; SD - standard deviation; Numerical results were presented as the arithmetic mean ± SD. The significance level was set at 0.05. Asterisks refer to statistically significant differences between the control group and the experimental group on the same sampling day at * p < 0.05; ** p < 0.01; A, B, C refer to statistically significant differences between day “0” and the consecutive sampling days in the experimental group at A – p < 0.05; B – p < 0.01; C – p < 0.001