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Table 1 Live cell count and hemolytic activity of strongly and weakly hemolytic B. hyodysenteriae strains during growth in broth and indication of time points chosen for hemolysin gene transcription studies

From: Differential expression of hemolysin genes in weakly and strongly hemolytic Brachyspira hyodysenteriae strains

B. hyodysenteriae strain Hours of incubation Hemolysin titer (mm) Live cell count (GFU50/mL) Time point chosen for hemolysin gene transcription study
during growth in BHIF-broth
Trial 1 Trial 2 Trial 3 Trial 1 Trial 2 Trial 3
B204 (strongly hemolytic) 0 0 0 0 3.75 4.25 4 no
14 0 0 0 5 5.5 4.75 no
24 0 0 0 6 6.25 5.75 yes
35 5 6 4 8 6.25 7.75 yes
40 6 6 5 7 6.75 6.75 yes
48 5 4 5 9.75 9.75 9 no
55 2 2 2 9.75 9.75 9.75 no
62 1 1 1 8.25 8.5 8 no
84 0 0 0 7.75 7.75 7.5 yes
G423 (weakly hemolytic) 0 0 0 0 3.75 4.75 4.75 no
17 0 0 0 4 4.25 4.25 no
25 0 0 0 5 4.75 5 no
41 0 0 0 5.5 5 5.5 no
47 0 0 0 5.25 6 5.5 no
53 0 0 0 6.25 6 6 yes
63 0 0 0 8.25 8.5 8 yes
73 0 0 0 8.5 8.25 8.5 no
87 0 0 1 8.5 8.5 8.5 no
92 0 1 1 9.5 9.5 9.5 no
99 1 1 1 9.5 8.75 9.5 no
110 3 2 3 9.5 9.5 9.5 yes
117 3 3 3 7.5 8 8.25 No
136 3 3 3 8.5 8.5 8.25 yes
147 4 2 4 4 6.5 1 no
160 3 3 3 4 6.25 1 no