Table 1 Live cell count and hemolytic activity of strongly and weakly hemolytic B. hyodysenteriae strains during growth in broth and indication of time points chosen for hemolysin gene transcription studies
B. hyodysenteriae strain | Hours of incubation | Hemolysin titer (mm) | Live cell count (GFU50/mL) | Time point chosen for hemolysin gene transcription study | ||||
|---|---|---|---|---|---|---|---|---|
during growth in BHIF-broth | ||||||||
Trial 1 | Trial 2 | Trial 3 | Trial 1 | Trial 2 | Trial 3 | |||
B204 (strongly hemolytic) | 0 | 0 | 0 | 0 | 3.75 | 4.25 | 4 | no |
14 | 0 | 0 | 0 | 5 | 5.5 | 4.75 | no | |
24 | 0 | 0 | 0 | 6 | 6.25 | 5.75 | yes | |
35 | 5 | 6 | 4 | 8 | 6.25 | 7.75 | yes | |
40 | 6 | 6 | 5 | 7 | 6.75 | 6.75 | yes | |
48 | 5 | 4 | 5 | 9.75 | 9.75 | 9 | no | |
55 | 2 | 2 | 2 | 9.75 | 9.75 | 9.75 | no | |
62 | 1 | 1 | 1 | 8.25 | 8.5 | 8 | no | |
84 | 0 | 0 | 0 | 7.75 | 7.75 | 7.5 | yes | |
G423 (weakly hemolytic) | 0 | 0 | 0 | 0 | 3.75 | 4.75 | 4.75 | no |
17 | 0 | 0 | 0 | 4 | 4.25 | 4.25 | no | |
25 | 0 | 0 | 0 | 5 | 4.75 | 5 | no | |
41 | 0 | 0 | 0 | 5.5 | 5 | 5.5 | no | |
47 | 0 | 0 | 0 | 5.25 | 6 | 5.5 | no | |
53 | 0 | 0 | 0 | 6.25 | 6 | 6 | yes | |
63 | 0 | 0 | 0 | 8.25 | 8.5 | 8 | yes | |
73 | 0 | 0 | 0 | 8.5 | 8.25 | 8.5 | no | |
87 | 0 | 0 | 1 | 8.5 | 8.5 | 8.5 | no | |
92 | 0 | 1 | 1 | 9.5 | 9.5 | 9.5 | no | |
99 | 1 | 1 | 1 | 9.5 | 8.75 | 9.5 | no | |
110 | 3 | 2 | 3 | 9.5 | 9.5 | 9.5 | yes | |
117 | 3 | 3 | 3 | 7.5 | 8 | 8.25 | No | |
136 | 3 | 3 | 3 | 8.5 | 8.5 | 8.25 | yes | |
147 | 4 | 2 | 4 | 4 | 6.5 | 1 | no | |
160 | 3 | 3 | 3 | 4 | 6.25 | 1 | no | |