A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

BMC Veterinary Research

Table 4 Primers used in the study

	Specific primer sequence(5′ → 3′)	Restriction sites	Amplification site	Fragment size
CSFV	CSFV-E2-F:5′-TGACTCTAGATATTTGGCATCATTGCATAAGGGGG-3′	XbaI	193–530	338 bp
CSFV	CSFV-E2-R:5′-TGACGGTACCGATCTTCATTTTCCACTGTGGTGG-3’	KpnI	193–530	338 bp
JEV	JEV-EDIII-F:5′-TGACGATATCCACCTGAAATGCAGGCTAAAAATGG-3’	EcoRV	849–1304	455 bp
JEV	JEV-EDIII-R:5′-TGACAAGCTTGAATACCCCTCCAATGGAGCC-3’	HindIII	849–1304	455 bp
PRRSV	PRRSV-N-F:5′-TGACGGATCCATGCCAAATAACAACGGCAGAC-3’	BamHI	1–372	372 bp
PRRSV	PRRSV-N-R:5′-TGACCTCGAGTCATGCTGAGGGTGGTGTT-3’	XhoI	1–372	372 bp
PPV	PPV-VP2-F:5′-TGACGGATCCAGCGCAACCAGTCCG-3’	BamHI	amino acids 156–438	849 bp
	PPV-VP2-R:5′-TGACAAGCTTCATATTGCTTTTACCACCAATCGGA-3’	HindIII	amino acids 156–438	849 bp

ISSN: 1746-6148