Skip to main content

Table 4 Primers used in the study

From: A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

 Specific primer sequence(5′ → 3′)Restriction sitesAmplification siteFragment size
CSFVCSFV-E2-F:5′-TGACTCTAGATATTTGGCATCATTGCATAAGGGGG-3′XbaI193–530338 bp
CSFV-E2-R:5′-TGACGGTACCGATCTTCATTTTCCACTGTGGTGG-3’KpnI
JEVJEV-EDIII-F:5′-TGACGATATCCACCTGAAATGCAGGCTAAAAATGG-3’EcoRV849–1304455 bp
JEV-EDIII-R:5′-TGACAAGCTTGAATACCCCTCCAATGGAGCC-3’HindIII
PRRSVPRRSV-N-F:5′-TGACGGATCCATGCCAAATAACAACGGCAGAC-3’BamHI1–372372 bp
PRRSV-N-R:5′-TGACCTCGAGTCATGCTGAGGGTGGTGTT-3’XhoI
PPVPPV-VP2-F:5′-TGACGGATCCAGCGCAACCAGTCCG-3’BamHIamino acids 156–438849 bp
 PPV-VP2-R:5′-TGACAAGCTTCATATTGCTTTTACCACCAATCGGA-3’HindIII