Fig. 2
Sensitivity of the LFD-RPA assay and conventional RT-PCR using 10-fold serial dilutions of standard control plasmids at concentrations ranging from 1 × 109–1 × 101 copies/μL; previously tested PDCoV negative samples was used as a negative control. The top line on the lateral flow dipstick is the control line, and the next line is the test line. a Standard control plasmids at concentrations ranging from 1 × 109–1 × 101 copies/μL. b Standard control plasmids at concentrations ranging from 1 × 109–1 × 101 copies/μL (lanes 1–9), and N is a negative control. M is a DNA marker