Fig. 5

Cytokines levels in ten mice immunized with the S. Choleraesuis vaccines. IFN-γ (a) and IL-4 (b) in spleens 7 d after the booster dose were assayed with an EILSA kit. A PBS control were also included. (c) the ratio of IFN-γ to IL-4. The significant differences between groups of each strain was indicated. The assay was performed in duplicate and repeated at least 3 times. *, P < 0.05; ** P < 0.01, for strains rSC0011, rSC0012, rSC0018 with either pYA3493 or pS-SaoA compared each other; #, P < 0.05; ##, P <  0.01, for the strains rSC0011, rSC0012, rSC0018 with pS-SaoA compared to these strains with pYA3493 respectively at 7 d and 14 d post immunization