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Table 2 The elution gradient has the following parameters: HPLC gradient, the flow is kept constant at 1 ml/min

From: Serum amino acid profile in 51 dogs with immunosuppressant-responsive enteropathy (IRE): a pilot study on clinical aspects and outcomes

Time (min)%A%B%C
0.010000
0.59910
18.09550
19.09190
29.583170
33.006040
3610000
5010000
  1. A aqueous buffer, B acetonitrile HPLC grade, C water HPLC grade