Table 2 The elution gradient has the following parameters: HPLC gradient, the flow is kept constant at 1 ml/min
| Time (min) | %A | %B | %C |
|---|---|---|---|
| 0.0 | 100 | 0 | 0 |
| 0.5 | 99 | 1 | 0 |
| 18.0 | 95 | 5 | 0 |
| 19.0 | 91 | 9 | 0 |
| 29.5 | 83 | 17 | 0 |
| 33.0 | 0 | 60 | 40 |
| 36 | 100 | 0 | 0 |
| 50 | 100 | 0 | 0 |
| Time (min) | %A | %B | %C |
|---|---|---|---|
| 0.0 | 100 | 0 | 0 |
| 0.5 | 99 | 1 | 0 |
| 18.0 | 95 | 5 | 0 |
| 19.0 | 91 | 9 | 0 |
| 29.5 | 83 | 17 | 0 |
| 33.0 | 0 | 60 | 40 |
| 36 | 100 | 0 | 0 |
| 50 | 100 | 0 | 0 |