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Table 1 The variation coefficients of intra-assay and inter-assay reproducibility of the real-time RT-PCR

From: Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay

Sample

Intra-assay

Inter-assay

Mean CT ± SD

CV(%)

Mean CT ± SD

CV(%)

1

8.03 ± 0.19

2.37

8.75 ± 0.20

2.29

2

10.73 ± 0.25

2.33

10.52 ± 0.19

1.81

3

13.94 ± 0.20

1.43

14.11 ± 0.23

1.63

4

17.69 ± 0.11

0.62

17.71 ± 0.18

1.02

5

20.82 ± 0.16

0.77

20.65 ± 0.19

0.92

6

24.12 ± 0.28

1.16

24.32 ± 0.21

0.86

7

27.09 ± 0.33

1.22

27.94 ± 0.26

0.93

8

29.94 ± 0.23

0.77

30.11 ± 0.13

0.43

9

33.25 ± 0.82

2.47

34.35 ± 0.73

2.13

  1. CV Coefficient of variation
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BMC Veterinary Research

ISSN: 1746-6148

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