Table 1 Primer/probes used in this study
Assay | Primer | Sequence 5′–3′ | Amplicon size (bp) | Tm (°C) | Target | Reference |
|---|---|---|---|---|---|---|
First round PCR | DFA | GAYTTYGCNAGYYTNTAYCC | 315 | 42 | DNA polymerase | [10] |
ILK | TCCTGGACAAGCAGCARNYSGCNMTNAA | |||||
KG1 | GTCTTGCTCACCAGNTCNACNCCYTT | |||||
Second round PCR | TGV | TGTAACTCGGTGTAYGGNTTYACNGGNGT | 222 | DNA polymerase | ||
IYG | CACAGAGTCCGTRTCNCCRTADAT | |||||
real time quantitative PCR (qPCR) | BoHV-2 Pol F | TGCTACGGTCACCACCATAG | 105 | 54 | DNA polymerase | this study |
BoHV-2 Pol R | TCCGGAAAGTCCATCACGAG | |||||
BoHV2 Pb | [FAM] CACTCGCGGTGGGTCAGCTTTGACG [BHQ1] |