Table 1 Primers and probes used in this study
Name | Sequence (5′ to 3′) | Amplicon size |
|---|---|---|
F1 | 5′-GAGCCCATTTTCTACTGACTCTAACACTGAC | 252 |
F2 | 5′-AGCCCATTTTCTACTGACTCTAACACTGAC | 251 |
F3 | 5′-GCCCATTTTCTACTGACTCTAACACTGACGT | 250 |
F4 | 5′-CCCATTTTCTACTGACTCTAACACTGACGT | 249 |
F5 | 5′-TTCTACTGACTCTAACACTGACGTTGAAGAAACC | 243 |
F6 | 5′-TCTACTGACTCTAACACTGACGTTGAAGAA | 242 |
F7 | 5′-ACTGACTCTAACACTGACGTTGAAGAAACCG | 239 |
F8 | 5′-CTGACTCTAACACTGACGTTGAAGAAACCG | 238 |
F9 | 5′-GAGATAGTGACCGATACTACTGCTGATCTTGAG | 152 |
F10 | 5′-AGATAGTGACCGATACTACTGCTGATCTTG | 151 |
F11 | 5′-AGATAGTGACCGATACTACTGCTGATCTTGA | 151 |
F12 | 5′-ATAGTGACCGATACTACTGCTGATCTTGAG | 149 |
R | 5′-Biotin -GTTCGTGAATAACAATACGCAAACAGGAAG | / |
Probe 1 | 5′-FITC-AAGCGCCAGCAGTGGAGTTAGAAGCGAGCG [THF]TACACCACCAACAGA-DDC | / |
Probe 2 | 5′-FITC-TCAAGTAGAGCCGACTAAAACTCAGCCGAA [THF]CCAGCATCTAACACG-DDC | / |
AlgF1 | 5′-TCAGAGAAAGTTGAGCTAACGATT | 568 |
AlgR1 | 5′-CATCGTCGCCTGAAGTCGCTGT | |
16S rRNA-F | 5′-AGAGTTTGATCCTGGCTCAG | / |
16S rRNA-R | 5′-TACGGTTACCTTGTTACGACTT |