Fig. 5

Phagocytosis and ROS-production capacity of camel monocyte subsets. Separated camel leukocytes were labeled with monoclonal antibodies to CD14 and MHCII. Labeled cells were incubated with heat killed FITC-labeled S. aureus bacteria. After gating on camel monocyte subsets (mo-I, mo-II, mo-III) the percentage of FITC-positive, phagocytosis-positive cells (A) and the mean fluorescence intensities (MFI) of phagocytosis-positive cells (B) was determined for each subset (means ± SEM). (C) Parallel setups of labeled camel leukocytes loaded with the ROS-sensitive dye dihydrorohdamin-123 (DHR-123) were stimulated with heat killed S. aureus bacteria. The reactive oxygen-dependent generation of rhodamin 123 was recorded flow cytometrically for gated camel monocyte subsets as the mean cellular fluorescence (ROS MFI, mean ± SEM, n = 21 animals.). Differences between groups were calculated using the one-way ANOVA and were considered significant (*) if p < 0.05