Fig. 2
From: Two novel protein chips for the detection of antibodies against porcine parvovirus
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12917-020-02280-z/MediaObjects/12917_2020_2280_Fig2_HTML.png)
Determination of the optimal spot concentration. a and b Dot sequence order of both protein chips. c and d Optimization of the antigen of both protein chips. e and f The antigen diagnostic concentration of the visible protein chip microarray is optimized. The amount of 0.3 mg/ml can be used as a diagnostic concentration after gray value analysis. The antigen diagnostic concentration of the Cy3-labeled protein chip diagnostic microarray is optimized. The amount of 0.1 mg/ml can be used as a diagnostic concentration