Skip to main content

Table 1 Haemodynamic parameters in control (C) and methylene blue groups (MB5 and MB20)

From: Assessment of effects of methylene blue on intestinal ischemia and reperfusion in a rabbit model: hemodynamic, histological and immunohistochemical study

Variables Group Basaline Ischemia R60 R120 R240 R360 Reperfusion
HR (bpm) C 207 ± 32 a 175 ± 41a 173 ± 31 178 ± 27 184 ± 28 190 ± 28 179 ± 29 ♯,
MB5 200 ± 18 a 182 ± 16 a,c 197 ± 28 209 ± 25 213 ± 24 216 ± 20 207 ± 26 c ♯,†
MB20 199 ± 23 a 176 ± 23 a,c 183 ± 26 198 ± 29 194 ± 37 200 ± 34 190 ± 30 c ,†
RR (rpm) C 35 ± 9 32 ± 8 36 ± 12 328 3510 3510 34 ± 10 ♯,
MB5 37 ± 10 35 ± 5 c 41 ± 8 436 4810 4610 43 ± 9 c
MB20 34 ± 7 33 ± 8 c 35 ± 8 4013 4212 4514 40 ± 12 c
MAP (mmHg) C 64 ± 6 a,b 41 ± 13 a 41 ± 11 42 ± 11 41 ± 10 38 ± 11 40 ± 10 b ♯,
MB5 66 ± 15 a 46 ± 13 a,c 65 ± 15 58 ± 13 56 ± 13 55 ± 12 59 ± 14 c ♯,†
MB20 61 ± 14 ª,b 47 ± 10 a 50 ± 9 45 ± 9 45 ± 7 46 ± 8 47 ± 9b ,†
SAP (mmHg) C 84 ± 12 a,b 52 ± 15 a 56 ± 14 59 ± 12 56 ± 12 52 ± 13 56 ± 13 b ♯,
MB5 89 ± 23 a 60 ± 18 a,c 95 ± 21 84 ± 15 79 ± 14 75 ± 14 85 ± 18 c ♯,†
MB20 83 ± 19 a 62 ± 17 a 78 ± 17 70 ± 18 66 ± 14 69 ± 14 72 ± 17 ,†
DAP (mmHg) C 51 ± 6 a,b 35 ± 11 a 32 ± 9 33 ± 10 33 ± 8 30 ± 9 31 ± 9 b ♯,
MB5 52 ± 14 a 39 ± 11 a,c 49 ± 12 45 ± 11 42 ± 10 42 ± 10 45 ± 11 c ♯,†
MB20 45 ± 13 a,b 37 ± 10 a 35 ± 9 33 ± 7 35 ± 6 37 ± 9 36 ± 6 b †,
CI (mL/min/kg) C 177 ± 52 a,b 115 ± 53 a 124 ± 49 126 ± 28 110 ± 16 127 ± 27 123 ± 39 b ♯,
MB5 190 ± 33 a 103 ± 29 a,c 172 ± 47 191 ± 53 186 ± 51 183 ± 45 182 ± 50 c
MB20 189 ± 39 a 111 ± 32 a,c 185 ± 68 168 ± 56 165 ± 60 177 ± 73 175 ± 63 c
dPmx (mmHg/s) C 888 ± 363 a,b 488 ± 161 a 628 ± 266 605 ± 274 535 ± 158 450 ± 128 584 ± 250 b ♯,
MB5 941 ± 193 a 558 ± 237 a,c 1244 ± 357 1072 ± 253 930 ± 256 918 ± 194 1080 ± 314 c
MB20 931 ± 276 a 577 ± 140 a,c 1218 ± 403 916 ± 463 874 ± 390 1017 ± 405 1031 ± 442 c
SVI (mL/beat/kg) C 0.88 ± 0.31 a 0.68 ± 0.30 a 0.73 ± 0.32 0.71 ± 0.18 0.55 ± 0.17 0.62 ± 0.16 0.69 ± 0.26 ♯,
MB5 0.95 ± 0.15 a 0.54 ± 0.13 a,c 0.93 ± 0.34 0.98 ± 0.34 0.92 ± 0.30 0.89 ± 0.32 0.94 ± 0.32 c
MB20 0.98 ± 0.26 a 0.65 ± 0.20 a,c 1.00 ± 0.36 0.83 ± 0.20 0.82 ± 0.22 0.89 ± 0.25 0.91 ± 0.30 c
SVRI (mmHg/mL/min/kg) C 0.37 ± 0.10 a 0.40 ± 0.18 a,c 0.33 ± 0.13 0.30 ± 0.08 0.32 ± 0.07 0.25 ± 0.1 0.30 ± 0.10 c
MB5 0.33 ± 0.09 a 0.44 ± 0.15 a,c 0.34 ± 0.07 0.32 ± 0.06 0.30 ± 0.08 0.31 ± 0.09 0.33 ± 0.11 c ♯,†
MB20 0.31 ± 0.11 a 0.42 ± 0.13 a,c 0.26 ± 0.10 0.27 ± 0.06 0.32 ± 0.07 0.30 ± 0.19 0.28 ± 0.09 c
CaO2 (mL/dL) C 16.4 ± 1.2 a,b 14.2 ± 1.7 a 15.1 ± 1.6 14.8 ± 1.4 13.8 ± 0.9 12.1 ± 1.9 14.8 ± 2.1 b  
MB5 15.7 ± 1.8 a,b 14.7 ± 1.5 a 15.6 ± 1.5 14.8 ± 1.5 13.9 ± 1.7 13.5 ± 1.9 14.4 ± 2.1 b
MB20 16.1 ± 1.1 a,b 14.1 ± 2.5 a 13.8 ± 2.6 13.1 ± 2.2 12.7 ± 2.5 12.3 ± 2.7 13.3 ± 2.6 b
DO2 (mL/min/kg) C 29.3 ± 8.3 a,b 15.9 ± 8.8 a 19.2 ± 5.9 16.2 ± 4.2 14.7 ± 4.1 14.8 ± 3.0 16.4 ± 4.6 b ♯,
MB5 29.5 ± 7.9 a 15.7 ± 7.1 a,c 25.7 ± 6.4 27.0 ± 4.9 24.8 ± 6.7 20.0 ± 7.4 24.4 ± 6.3 c
MB20 30.1 ± 8.6 a 15.6 ± 4.5 a,c 28.5 ± 13.9 24.5 ± 5.6 21.9 ± 8.2 19.7 ± 8.5 23.6 ± 10.5 c
VVS (%) C 12 ± 2 a,b 22 ± 7 a 19 ± 5 21 ± 6 21 ± 5 24 ± 7 20 ± 5 b
MB5 10 ± 3 a 19 ± 6 a,c 15 ± 4 15 ± 6 13 ± 5 12 ± 3 14 ± 5 c ♯,†
MB20 12 ± 3 a,b 19 ± 5 a 17 ± 6 17 ± 8 19 ± 9 19 ± 10 18 ± 8 b
Lactate (mmol/L) C 2.8 ± 1.1 a,b 5.6 ± 1.8 a 7.7 ± 2.0 6.6 ± 2.4 6.0 ± 1.7 5.7 ± 1.9 6.9 ± 2.6 b
MB5 2.4 ± 1.0 a,b 4.9 ± 1.3 a, 5.5 ± 1.2 4.7 ± 1.7 4.3 ± 1.4 4.5 ± 1.4 4.8 ± 1.2 b ♯,†
MB20 2.8 ± 0.9 a,b 5.2 ± 1.9a 6.3 ± 2.1 6.4 ± 3.1 6.0 ± 3.0 5.5 ± 2.4 6.1 ± 2.4 b
Hemoglobine (gr/dL) C 12.3 ± 1.1 a,b 10.4 ± 1.4 a 10.9 ± 1.6 10.7 ± 1.5 9.2 ± 1.6 8.9 ± 1.4 10.3 ± 1.5 b  
MB5 11.8 ± 1.3 a,b 10.6 ± 1.1 a 11.7 ± 1.3 11.2 ± 1.5 10.1 ± 1.7 10.0 ± 1.4 10.9 ± 1.4 b  
MB20 12.2 ± 1.2 a,b 10.5 ± 1.8 a 10.8 ± 2.0 10.1 ± 1.7 9.9 ± 1.8 9.7 ± 1.9 10.3 ± 1.4 b  
  1. Legend: Data are expressed as mean ± SD. The reperfusion column represents the average value of the different periods of reperfusion
  2. a Significant difference (P < 0.05) between baseline and ischemia. b Significant difference (P < 0.05) between baseline and reperfusion. c Significant difference (P < 0.05) between reperfusion and ischemia
  3. ♯. Significant difference (P < 0.05) between Control and MB5 in reperfusion. Significant difference (P < 0.05) between Control and MB20 in reperfusion.
  4. †. Significant difference (p < 0.05) between MB5 and MB20 in reperfusion