Full genomic characterization of a porcine rotavirus strain detected in an asymptomatic piglet in Accra, Ghana

BMC Veterinary Research

Table 3 Primers and probes used in RT-PCR

Genome Segment	Protein	Primer/probe sequence	Predicted product size (bp)	Reference
7	NSP3	NSP3-F: ACCATCTWCACRTRACCCTCTATGAG NSP3-R: GGTCACATAACGCCCCTATAGC NSP3-p: FAM5’-AGTTAAAAGCTAACACTGTCAAA-3’MGB	87 bp	[32]
6	VP6	VP6F: GACGGVGCRACTACATGGT VP6R: GTCCAATTCATNCCTGGTGG	380 bp	[33]
2	VP2	VP2F1: ACAAGCGGCAAATGACTGTT VP2R1: GGTTGATGACTTGTCCACTCC	398 bp	This paper
11	NSP5	NSP5F1: ACAACGTCAACTCTTTCTGGA NSP5R1: TGCTTGAAGGTCGTGATTGC	232 bp	This paper
	SISPA	FR26RV-N: GCCGGAGCTCTGCAGATATCNNNNNN FR20RV: GCCGGAGCTCTGCAGATATC		[34]

ISSN: 1746-6148