Fig. 4

Protein expression of VEGFA (a, b), VEGFR1 (c, d) and VEGFR2 (e, f) in endometrial and myometrial bovine tissue during adenomyosis, determined by Western blotting. Data were normalized against GAPDH. Bars represent the mean ± SEM. Different letters (a, b) indicate statistically significant differences between healthy tissues (Control, n = 7) and tissues from stages 1–2 (n = 7), and stages 3–4 of adenomyosis extent (n = 7). P < 0.05 was considered as significant, and was determined using one-way ANOVA, followed by Bonferroni’s post-hoc test. Representative blots for VEGFA, VGFR1, VEGFR2 and GAPDH are shown below the graphs (g). MM: molecular weight marker