Table 2 Details of primers used in this study
From: Molecular evidence of Babesia in pet cats in mainland China
| gene | Primers | Sequences (5′ → 3′) | Tm | Amplicon (bp) | Reference |
|---|---|---|---|---|---|
| 18S rRNA | Piro1-S | F1: CTTGACGGTAGGGTATTGGC | 55 °C | 1400 | [27] |
| Piro3-AS | R1: CCTTCCTTTAAGTGATAAGGTTCAC | [27] | |||
| Piro-A | F2: ATTACCCAATMCBGACACVGKG | 55 °C | 407 | [27] | |
| Piro-B | R2: TTAAATACGAATGCCCCCAAC | [27] | |||
| P1 | F: AACCTGGTTGATCCTGCCAGTAGTCAT | 54 °C | 1700 | [20] | |
| P2 | R:GATCCTTCTGCAGGTTCACCTAC | [20] | |||
| ITS region | ITS-F | F: GAGAAGTCGTAACAAGGTTTCCG | 54 °C | 1100 | [20] |
| ITS-2 | R: ACAATTTGCGTTCAATCCCA | [20] |
