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Table 3 Detection of H6N2 antibodies in three field scenarios using hemagglutination inhibition (HI) assays and ELISAs

From: Continuing evolution of H6N2 influenza a virus in South African chickens and the implications for diagnosis and control

 A
Vaccinated chicken, no field challenge*
B
Vaccinated chicken with field challenge**
C
Non-vaccinated chicken with field challenge***
Serum titrationELISAHI log2 titreELISAHI log2 titreELISAHI log2 titre
BioChek titre1IDEXX S/N22002
H6N2Ag
1998
H6N8Ag
2016H6N2AgBioChek titre1IDEXX S/N22002
H6N2Ag
1998
H6N8Ag
2016
H6N2Ag
BioChek titre1IDEXX S/N22002
H6N2Ag
1998
H6N8Ag
2016
H6N2Ag
0667 (1)0.8884910,755(7)0.15128125753 (4)0.238512
1:1336 (0)0.567277383 (5)0.13107113910 (3)0.125210
1:2124 (0)0.726264850 (4)0.4997102296 (2)0.20429
1:450 (0)0.715153802 (3)0.4486101494 (2)0.13328
1:81 (0)0.744042155 (2)0.17758869 (1)0.23207
1:161 (0)0.793031107 (1)0.21646556 (0)0.34206
1:321 (0)0.83303556 (0)0.27524331 (0)0.28105
1:641 (0)0.73202253 (0)0.48423131 (0)0.40004
1:1281 (0)1.110196 (0)0.6631382 (0)0.56003
1:2561 (0)1.0700112 (0)0.872031 (0)0.65003
  1. * Prime-boost vaccinated with commercial inactivated whole virus H6N2 vaccine (strain W-04/2002)
  2. ** Vaccinated once with commercial H6N2 inactivated vaccine (strain W-04/2002), challenged with H6N2 strain H44954/2016
  3. *** Challenged with H6N2 strain H44954/2016
  4. 1Titer group in brackets
  5. 2Sample to negative ratio
  6. Ag-Antigen
  7. Positive serological test results are highlighted in boldface