Table 7 Primer sequences, reference sequences, covered regions and positions in the mRNAs, and expected amplicon sizes for both the target and reference genes used in the qPCR analysis
Gene symbol | Primer sequences | Reference sequence | Covered region / Position | Amplicon, base pairs |
|---|---|---|---|---|
B4GALT1 | FW-5’CTGTGTCTCGCCCAAATGCT3’ RW-5’AGGTGAGTGAGTTCAAACCATCAG3’ | HQ700335.1 | Ex5fw/1011–1030 Ex6rw/1134–1157 | 147 |
LALBA | FW-5’GATGACATTGTGTGTGCCAAGA3’ RW-5’AGTGCCACTGATCCAGCTTCTC3’ | NM_001285635.1 | Ex3fw/330–351 Ex4rw/408–429 | 100 |
ST3GAL5 | FW-5’CCTGAACCAGTTTGATGTCG3’ RW-5’GGTGCACCTTCTGGATAAGTC3’ | KF055858.2 | E4fw/655–673 E5rw/745–765 | 111 |
ST6GAL1 | FW-5’GAGCTGTGGGACATCATTCAAG3’ RW-5’CACACAGTGACATCATAATGGCAAT3’ | HQ709167.1 | E5fw/1065–1086 E6rw/1140–1164 | 100 |
ATP5B | FW-5’TTTGGACTCCACGTCTCGCATC3’ RW-5’TCCTGGAGGGATTTGTAGTCCTG3’ | NM_175796.2 | Ex8fw/1219–1240 Ex9rw/1304–1326 | 108 |
EIF2B2 | FW-5’CCGTTCCCATTATGCTCAACTCCAG3’ RW-5’TCCGTTGTCCCTTCCAGTTCCAC3’ | NM_001015593.1 | Ex3fw/395–419 Ex3-4rw/453–475 | 81 |
SDHA | FW-5’ACGATTACTCCAAGCCCATCCAG3’ RW-5’AACGTAGGAGAGCGTGTGCTTC3’ | NM_174178.2 | Ex14fw/1825–1847 Ex14rw/1883–1904 | 80 |
POLR2A | FW-5′ AATGGAAGCATGTCAATGAGGACTCTC3’ RW-5′ CACAGGCAGCACAGTGACGATC3’ | NM_001206313.1 | Ex5fw/602–628 Ex5rw/744–765 | 164 |
TBP | FW-5’AACAGGTGCTAAAGTCAGAGCAG3’ RW-5′ GGAGAACACAGCAGCCATTACG3’ | NM_001075742.1 | Ex8fw/1254–1276 Ex9rw/1334–1355 | 102 |
UXT | FW-5’TGTGGCCCTTGGATATGGTT3’ RW-5’GGTTGTCGCTGAGCTCTGTG3’ | NM_001037471.2 | Ex5fw/300–319 Ex5-6rw/381–400 | 101 |
RPS9 | FW-5’CCTCGACCAAGAGCTGAAG3’ RW-5’CCTCCAGACCTCACGTTTGTTC3’ | NM_001101152.1 | Ex2fw/128–146 Ex3rw/170–191 | 54 |