Identification of the complete coding cDNAs and expression analysis of B4GALT1, LALBA, ST3GAL5, ST6GAL1 in the colostrum and milk of the Garganica and Maltese goat breeds to reveal possible implications for oligosaccharide biosynthesis

Table 6 Primer sequences, reference sequences, position in the reference, T.a. and amplicon size of target genes

Gene symbol	Primer sequences	Reference sequence	Position	T.a.	Amplicon base pair
B4GALT1	FW-5’TAAAGCGGCGGCGGGAAGAT3’ RW-‘5AATGAGAGGGACCAGCCCA3’	NM_177512.2	166–185 1460–1478	57	1313
B4GALT1	FW-5’GTTTCTCAGCATCAATGGATTTCC3’ RW-5’GCTTTGATTCTTTGGGGTGA3’	NM_177512.2	1083–1106 1652–1672	58	590
ST3GAL5	FW-5’CCCGCTCCCTAATATGCGAA3’ RW-5’TGCGATCAGGATCCATGTAA3’	NM_205807	30–49 342–361	58	332
ST3GAL5	FW-5’AATGCCAAGTGACCACAGC3’ RW-5’CGACATCAAACTGGTTCAGG3’	NM_205807	129–147 684–704	58	460
ST3GAL5	FW-5’CCTGAACCAGTTTGATGTC3’ RW-5’CAGCATTGGAAGCACAGAGT3’	NM_205807	684–704 1369–1389	58	705
ST6GAL1	FW-5’TCCTGAGAAGAATGAGCCTTG3’ RW-5’AGTGAGACAGGAGGCTCTGG3’	NM_177517	15–35 1597–1617	56	1593
ST6GAL1	FW-5’TGAAGTACCTCAACCTCGGCA3’ RW-5’GGGAAAGGTGGATCTTGCG3’	NM_177517	1307–1329 1677–1696	58	379

ISSN: 1746-6148