Enolase and dipeptidyl peptidase IV protein sub-unit vaccines are not protective against a lethal Streptococcus suis serotype 2 challenge in a mouse model of infection

Table 1 Bacterial strains and plasmids used in this study

Strains/plasmids	General characteristics	Source/reference
Escherichia coli
TOP10	F⁻ mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Str^R) endA1 nupG	Invitrogen
BL21	F⁻ompT hsdS_B (r_B⁻, m_B⁻) gal dcm rne131 (DE3)	Invitrogen
Streptococcus suis
31533	Virulent European serotype 2 ST1 strain isolated from pig with meningitis	(42)
ΔdppIV	Isogenic mutant strain derived from P1/7 strain. In frame deletion of dppIV	(20)
Group B Streptococcus
COH-1	Capsular type III strain isolated from an infant with bacteremia	(40)
Plasmids
pET-32a	Ap^r, pBR322 ori, T7 promotor	Novagen
pBAD/thio	Ap^r, pUC ori, P_BAD promotor	Invitrogen
pET-32aEnolase	pET-32a carrying eno gene for protein production	This work
pBAD/thioDPPIV	pBAD/thio-TOPO carrying dppIV gene for protein production	This work

ISSN: 1746-6148